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Several amino acids and derivatives were also identified as substrates for growth during exometabolomics experiments (Table S12–13). This is consistent with the use of peptides and other amino acid-containing substrates like yeast extract and peptone by T. Bonchosmolovskayae [27] and other Chloroflexota [34, 35, 62, 68]. Cryo-ET also revealed other structural features that are distinct from known organohalide-respiring Dehalococcoidia. Members of the order Dehalococcoidales range in cell shape from irregular discs (Dehalococcoides mccartyi [44, 45]) to irregular or slightly flattened cocci (Dehalogenimonas spp [6, 45, 46]).
Together, these data suggest that, like other Chloroflexota, fatty acids and their precursors and/or derivatives play an important role in sustaining growth of Tepidiforma species [27]. A Cladogram inferred from the Dehalococcoidia phylogenomic tree serving as evolutionary hypothesis for ancestral character state reconstruction. The presence of three sets of conserved genes is indicated on the cladogram.
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ARM, AB, AH, and EIT performed TEM, cryo-electron tomography, visualized results, and interpreted data. MP and BPH wrote, reviewed, and edited the initial drafts of the paper, and all authors critically reviewed and contributed to the final version of the paper. Sediment and microbial mat samples were collected with sterile spatulas and spoons, and samples were homogenized in pre-sterilized aluminum pans. Homogenized samples were dispensed into 15 ml polypropylene tubes and transported to the lab in the dark without temperature control.